Journal: MethodsX

Article Title: Around the ThermoClock : A precision automated temperature control system for Ex Vivo circadian studies

doi: 10.1016/j.mex.2025.103717

Figure Lengend Snippet: ThermoClock system overview schematics. The microcontroller (Arduino DUE) receives the desired temperature vector, T desired ( t ) , which contains setpoints of each temperature module, T desired , i ( t ) . It also the measured temperature array, T measured ( t ), which contains real-time temperature readings from each module, T measured , i ( t ) . The microcontroller calculates the error array, ( t ) , which is the input to the proportional-integral-derivative (PID) control operating under the same gains for every module. The PID control output PWM signals, u i ( t ) , which are sent to individual bipolar junction transistors (BJTs). BJTs regulate the current output, i i ( t ) , to the heating pads, thereby controlling their temperatures and that of the well plates placed on top. The actual temperature of each module is measured using a resistance temperature detector (RTD), then sent back to the microcontroller for calculation and for logging onto a microSD card.

Article Snippet: Platinum RTD Sensor - PT100 - 3 Wire 1 m long , 5 , Adafruit , 59.75 , .

Techniques: Plasmid Preparation, Control

Journal: MethodsX

Article Title: Around the ThermoClock : A precision automated temperature control system for Ex Vivo circadian studies

doi: 10.1016/j.mex.2025.103717

Figure Lengend Snippet: ThermoClock system overview schematics. The microcontroller (Arduino DUE) receives the desired temperature vector, T desired ( t ) , which contains setpoints of each temperature module, T desired , i ( t ) . It also the measured temperature array, T measured ( t ), which contains real-time temperature readings from each module, T measured , i ( t ) . The microcontroller calculates the error array, ( t ) , which is the input to the proportional-integral-derivative (PID) control operating under the same gains for every module. The PID control output PWM signals, u i ( t ) , which are sent to individual bipolar junction transistors (BJTs). BJTs regulate the current output, i i ( t ) , to the heating pads, thereby controlling their temperatures and that of the well plates placed on top. The actual temperature of each module is measured using a resistance temperature detector (RTD), then sent back to the microcontroller for calculation and for logging onto a microSD card.

Article Snippet: Adafruit PT100 RTD Temperature Sensor Amplifier - MAX31865 , 5 , Adafruit , 74.75 , .

Techniques: Plasmid Preparation, Control

Journal: MethodsX

Article Title: Around the ThermoClock : A precision automated temperature control system for Ex Vivo circadian studies

doi: 10.1016/j.mex.2025.103717

Figure Lengend Snippet: Connecting the PCB to the sensors, heating pads, and microSD card. (A) ThermoClock Wiring (Refer to for individual component positions; Abbreviations on PCB: Pad 1, 2, 3,… = heating pad 1, 2, 3, …; TR1, 2, 3,… = BJT 1, 2, 3,…; R1,2,3,…=220 ohm resistor 1, 2, 3…; Sensor1, 2, 3,…= RTD 1, 2, 3,….; SPI = (Arduino DUE) serial peripheral interface; LCD = liquid crystal display; 5VReg1= 5 V linear voltage regulator; C1 = 0.33 µF capacitor; C2 = 0.1 µF capacitor; abbreviations adjacent to the connectors (labelled G) correspond to pins on the SD and MAX31685 breakout boards). (B) Fully wired PCB and Arduino for operating five temperature modules with zoom in on MAX31865 breakout boards configuration.

Article Snippet: Adafruit PT100 RTD Temperature Sensor Amplifier - MAX31865 , 5 , Adafruit , 74.75 , .

Techniques: